human normal colon epithelial cells ncm460  (Procell Inc)

Journal: The Turkish Journal of Gastroenterology

Article Title: Matrine Alleviates Oxidative Stress and Inflammation in Colon Cancer by Activating the Nrf2 Pathway

doi: 10.5152/tjg.2025.24438

Figure Lengend Snippet: MAT inhibited viability, oxidative stress, and inflammation of colon cancer cells by activating the Nrf2 pathway. (A) Cell counting kit-8 assay was used to detect the cell viability of NCM460 cells and HT-29 cells. n = 6. (B) The transfection efficiency of si-Nrf2 was confirmed by qPCR. n = 3. (C) Methylthiazolyldiphenyl-tetrazolium bromide assay was used to measure HT-29 cell viability. n = 6. (D) Flow cytometry analysis was employed to test the apoptosis of HT-29 cells. n = 3. (E) The activities of SOD and MDA in HT-29 cells were quantified. n = 6. (F) The levels of IL-6 and TNF-α were tested by ELISA. n = 6. (G) Western blot assays were used to determine the expression levels of Nrf2, KEAP1, NQO1, and HO-1 proteins in HT-29 cells. n = 3. Results were presented as mean ± SD. + P < .05 and ++ P < .01 vs. Control group; # P < .05 and ## P < .01 vs. MAT + si-NC group.

Article Snippet: Human CC cells HT-29 (BFN60800646) and human normal colon epithelial cells NCM460 (BFN608006385) were obtained from the American Type Culture Collection (USA).

Techniques: Cell Counting, Transfection, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control

Journal: Journal of Inflammation Research

Article Title: Supplementation of Glucosamine Selenium Ameliorates DSS-Induced Chronic Colitis in Mice via Affecting Gut Microbiota, Inhibiting Pyroptosis and Inactivating Chemokine Signaling Pathway

doi: 10.2147/JIR.S486751

Figure Lengend Snippet: GASe-DS alleviates DSS-induced cell apoptosis and inflammatory response via inactivating chemokine signaling pathway. ( A ) The effects of series of GASe-DS content on cell viability. ( B ) 1% GASe-DS and reparixin increase cell viability, while ATI-2341 TFA decreases cell viability of DSS-treated NCM460 cells. ( C ) Flow cytometry shows that GASe-DS and reparixin treatment decrease cell apoptosis, while ATI-2341 TFA increases cell apoptosis. ( D ) Administration of GASe-DS and reparixin decrease the levels of IL-12, IL-6 and TNF-α. ATI-2341 TFA promotes the expression of IL-10, IL-6 and TNF-α. ( E ) Western blot analysis reveals that administration of GASe-DS and reparixin promote the expression of ZO-1, Occludin and claudin-1, and ATI-2341 TFA downregulates ZO-1, Occludin and claudin-1 in NCM460 cells. ( F ) Administration of GASe-DS and reparixin downregulate the expression of CXCL1 and CXCL2, which is reversed by ATI-2341 TFA in NCM460 cells. *** p < 0.001, compared with the control group. ### p < 0.001, compared with the DSS group. & p < 0.05, && p < 0.01, and &&& p < 0.001, compared with the DSS + GASe-DS group.

Article Snippet: NCM460, normal human colon mucosal epithelial cells, were obtained from icell Bioscience Inc. (Shanghai, China) and cultured in DMEM medium (HyClone, USA) containing 10% FBS (Biosera, France), 1% streptomycin-penicillin (Beyotime Institute of Biotechnology) at 37°C in an incubator with 95% humidity and 5% CO 2 .

Techniques: Flow Cytometry, Expressing, Western Blot, Control